2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. The shift in emission peak is also automaticallyThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 005) Photometric PrecisionSpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. The SpectraMax M2 and M2e are versatile and reliable. Align the two slots on the bottle holder with the two pegs on the rail. 1) SpectraMax Glo Steady Luc Reporter Assay (6. 1. Table of Contents. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). SpectraMax Microplate Readers. Place the plate drawer transport lock on the end of the plate drawer. SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Read mode. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. Background signal was determined by adding 500 U/ml Catalase from Bovine Liver (Sigma-Aldrich, E3289) in one well to convert hydrogen peroxide into. As for each measurement, I. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 2. 1step assay with SpectraMax microplate readers. RP-3500 is a novel, orally bioavailable clinical. Fusion of liposomes was followed by content mixing of the RPLs and subsequent increase in fluorescence which was monitored in a SpectraMax M3 fluorescence plate reader (Molecular devices). The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. . SpectraMax Glo and DuoLuc. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. D8000C; R&D Systems, Minneapolis,. SoftMax Pro. 315 can't find zero order. For each extract concentration, percentage parasite viability and. Polyplex sizes and zeta potentials were measured with a Malvern Instruments Zetasizer (Nano-2590) at a scattering angle of 173°. 10. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. The ProcartaPlex 384-well format was specifically developed to increase sample throughput while maintaining the robustness of a 96-well assay. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. . 0nm increments. In the M2, M5 and Plus. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader. Add to Cart. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Shake intensity. Recommended SpectraMax iD3/iD5 reader settings. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $12,125. BioTek Synergy LX Multi-Mode Microplate Reader (FLD/Lumi) Accuris MR9600 SmartReader 96 and MW9600 Smartwasher 96 Bundle, 115V. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. Mitosox works fine in 96 wells. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. The SoftMax® Touch Software is installed in. Introduction. Learn more about its features, specifications, and applications in this user guide. book Page 1 Tuesday,. Multimode Reader Comparison Chart. The drawing shows a standard microplate of 15 mm height covered with a lid. We would like to show you a description here but the site won’t allow us. No accessories are needed and SoftMax® Pro. 1. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax® ABS Plus. Next to the pushbutton is a green LED. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 312 gain calibration failed. Tumorsphere formation assay and limiting dilution assay. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. To assess mast cell degranulation, β-hexosaminidase release was evaluated according to a method published by Dearman et al. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Plate Formats: 6, 12, 24, 48, 96, 384 wells. These solutions were used to measure the activity of the parasite lactate dehydrogenase (pLDH) enzyme in the cultures. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. FlexStation™ II. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Mitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer (SpectraMax ® M3; Molecular Devices, San Jose, CA, United States) at 540-nm emission and 580-nm extinction (Maciel et al. Aggregation of MDH was monitored as a function of light scattering at 360 nm over 30 min at 51°C in a SpectraMax M3 (Molecular Devices, United States) microplate spectrometer. 4. D 1 1. 2 release. Figure 2-23: Bottle holder To install the bottle holder: 1. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 6: bioNova científica, s. . The innovative and flexible design features. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. Molecular Devices SpectraMax M3 User Manual Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. For more information, please visit us at per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. For more sample throughput, the system can StakMax Handling Stacker Base Unit. 1) EarlyTox NucView 488 Assay (6. Brochure. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. No. 2. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Photometric accuracy. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. o For example: Ex: 485/Em 525/Cutoff 515 4. The positive control was treated with fresh RPMI-1640 (Lonza, Walkersville, MD). Description 1. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. 305 reference level saturation. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The sample was subsequently washed five times with 200 mL PBS. SpectraMax® ABS. SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. com. Note: In this user guide, all references to SpectraMax Multi-Mode. Neutrophils were stimulated as described in tissue-culture multi-well plates. FI assay. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9 SpectraMax® L (software version 5. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. Fluorescent Polarization: Solid black plates, as above. . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax ABS Plus reader utilizes Molecular . 303 unable to cal dark current. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 37) was ordered from Sigma Aldrich (St. HNE (EC 3. no. SpectraMax M3, M4, M5, and M5 e. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. NETosis Assays Using Immunostaining and Microscopy. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. Table 1. SpectraMax i3x Multi-Mode Detection Platform. Amplitude : 1. Cells were cultured for at least 10 days in sphere-forming medium. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Figure 1. • Tel. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 0200-0729. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. , contact Molecular. Antiretroviral efficacy was determined by measuring HIV reverse transcriptase (RT) activity in culture medium. SpectraMax M3, M4, M5, and M5 e. Light Source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes. Also refer to this Multimode Reader Comparison Chart. Our Price: $8,635. 4. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. Luciferase Detection Instrumentation Guide. 0 out of 5 $ 11,999. Supplier: Molecular Devices. 9000-0756. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. Spectral Range. SpectraMax M5/M5e/M4/M3/M2/M2e. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Stray light. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 313 reference gain check fail. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Accuris SmartDrop. The concentration of CTC was expressed as mg catechin equivalent per g of extract. When the user applies a set temperature of 37°C, the top is heated to 37. Examples: “Press carefully to engage,” “ Do not. ® reader to the computer. 00 . Page 12 SpectraMax MiniMax Imaging Cytometer Installation Guide 10. Use the 3. Published Dec 16, 2020 | Updated Dec 16, 2020. 2. 1. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. WARNING! The instrument weighs approximately 36 pounds (16. Molecular Devices SpectraMax® series microplate readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Plant material. . Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. spr SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 1. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Supplier: Molecular Devices. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. SpectraTest ABS1 Absorbance Validation Plate. ScanLater Western Blot Detection Cartridge. This user guide provides detailed instructions on how to perform various tests, interpret the results, and troubleshoot common issues. 과 같은 Parameter를 검사하여 평가할 수 있습니다. After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. Figure 6: Transcreener ADP2 FI assay principle. 4. Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. Cell Degranulation. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The fluorescence of the reaction was read every 3 min (λ excitation = 485 nm, λ emission = 538 nm) for up to 90 min at constant 37 °C with the SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Activity I Want To Make Friends… And I Need Your Help. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Examples: “Press carefully to engage,” “ Do not press down. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Both readers feature an 8-channel read head for fast reads and. SpectraMax® Paradigm® Multi-Mode Detection Platform. 2. 2. The innovative and flexible design features. Detection modalities are shown in Table 1-1. Recertification is recommended every two years. Note: In this user guide, all references to SpectraMax Multi-Mode. In addition, the total flavonoid content was determined using the aluminum chloride method. 3: The cuvette chamber. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Additionally, SoftMax® Pro Software automatically identifies the optimal excitation and emission wavelengths via the Spectral Optimization Wizard. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Fluorescence (FL). The fluorescence intensity of the cells was detected using a SpectraMax M3 florescence microplate reader (Molecular Devices) with excitation and emission wavelengths of 485 and 535 nm, respectively. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader. Abstract. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Plate chamber design of the SpectraMax category 1 microplate readers. Choose cables that meet the requirement described in this appendix. spr * SpectraMax i3(x) SpectraTest ABS2 v1. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. STAKMAX. Dual monochromators increase performance and assay flexibility. The guide also covers the software features, the specifications, and the accessories of the systems. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Application note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. LINKS to Craig…His YouTube is @BrickArmy and his business is: is COBI’s website: set in this video is #2535 M. Generate more data points without the need to dilute. ; The top 3 importers of Spectramax are India with 365. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® iD3 Multi Mode Microplate . User Manual – SpectraMax M3 M4 M5 M5e. The optical densities (OD) were determined using the SpectraMax ® Plus 384 Microplate Reader (SpectraMax M3; Molecular Devices, SanJosé, CA, USA) at 450 nm and 660 nm [31,34]. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices Spectramax M3 Multi Mode Microplate Reader; Related products. 2 software (Sunnyvale, CA, USA). . The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 더 많은. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. ZERO BIAS - scores, article reviews, protocol conditions and moreF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Other technologies such as imaging. 7°C (gradient heating design). The Challenge. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5,. Unparalleled performance on a personalized platform. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Wavelength Range. spr SpectraMax Plus 384 SpectraTest ABS2 v1. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Multimode Reader Comparison Chart. e. Transfection with siRNA. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 9000-0756. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Trends within and amongst the times series, reaction half-lives, and maximum chemiluminescent intensities are discussed. AquaMax® 2000. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Moving a SpectraMax Multi-Mode Microplate Reader. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. The bacterial colony was mixed with. SpectraMax® M5e. book Page 1 Wednesday, July 7, 2010 2:09 PM When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. com. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 2 only) SpectraMax M-series Microplate readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Summary of Contents for Molecular Devices SpectraMax M2. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Filterless readers provide consistent results with steady temperature regulation. 1. At each time point, cells were incubated with CCK-8 reagent for 1 hour at 37 °C, followed by measuring the optical absorbance at 450 nm with the SpectraMax M3 microplate reader (Molecular Devices. Call 516-939-0888 to check current. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. The supernatant was collected after 20 min of incubation in the dark. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. 00 . Sphere-like cells were subcultured into single-cell suspension, and cells of ≥90% viability were used for sphere formation studies. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. 0 out of 5 $ 2,999. Italic and boldface type are used for emphasis. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). When a feature or capability applies to only certain readers, this exception is noted. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Fluorescence of the samples were read using a SpectraMax M3 instrument with an excitation set to 355 nm and emission set to 460 nm. Bottom-reading is usually recommended for cell-based assay with adherent cells. The absorbance was measured at 765 nm using SpectraMax M3 Microplate Reader with SoftMax Pro 7. 0 mm hex key. Cables. com. Multimode Reader Comparison Chart. The % parasite viability, as indicated by the pLDH activity in the treated wells compared with the untreated controls, was calculated. For a complete list of the instruments supported by this release of the8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The exactly same condition without CCK-8 solution was used to subtract background signal that is absorbed by cell medium or natural product. Impact of fix:Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Find regional support. Description 1. spr SpectraMax iD3 SpectraTest ABS2 v1. Also refer to this Multimode Reader Comparison Chart. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Wafi’s main research topic aims to set up a pharmacodynamic model comparing antibiotic activities against biofilms and examine in parallel their effects on viability and biofilm mass. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. 0. Page 28 Chapter 5: Read Modes and Read Types 1. The detection of intracellular ROS and mitochondrial membrane potential SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Example: [Drawer]. 4. Cells were treated with potassium bromate (30 mM) to provide a pro-oxidant source required to lower the glutathione ratio, which was used as a. It does not work as expected. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 2. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 1 & 7. The SpectraTest ABS1 Validation Plate package. I don’t care what you do or where…The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a series of multi-detection, dual-monochromator, multi-mode instruments with a triple-mode cuvette port and 6 to 384-well microplate reading capabilities. reader in the visible range between 405–750 nm with NIST and NMI. Microtitre plates were incubated for 2 h at 37°C in the dark. The staphyloxanthin intermediates present in collected supernatant were quantified by measuring at different wavelengths using multi-label reader (Spectramax M3, USA). Choose cables that meet the requirement described in this appendix. To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. All procedures completely complied with the manufacturer’s instructions. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. e. Add to Cart. Then, adding 2 M sulfuric acid, the reaction was stopped, and the absorbance at 450 nm was determined using a SpectraMax M3 microplate reader (Molecular. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 21. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 0 OD for earlier models. Multimode Readers Comparison Chart. Keywords: Spectramax M2 Systems, Spectramax M2e Systems, Spectramax M2/m2e Readers, Pathcheck Sensor, Spectratest Abs1 Absor Jun 8th, 2023SpectraMax M3, M4,. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. SpectraMax i3 Multi-Mode Platform A flexible, user-upgradeable microplate detection system Key Features The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Please contact Tatiana to reserve the plate reader time: tnl1@psu. 1. .